{"product_id":"protein-expression-in-mammalian-cells-methods-and-protocols-paperback","title":"Protein Expression in Mammalian Cells: Methods and Protocols - Paperback","description":"\u003cp\u003eby \u003cb\u003eJames L. Hartley\u003c\/b\u003e (Editor)\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e1. Why Proteins in Mammalian Cells?\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e James L. Hartley\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e2. \u003c\/b\u003e\u003cb\u003eLarge Scale Transfection of Mammalian Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Lucia Baldi, David L. Hacker, Carine Meerschman, and Florian M. Wurm\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e3. \u003c\/b\u003e\u003cb\u003eSelection of High Expressing Mammalian Cells by Surface Display of Reporters\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Christine T. DeMaria\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e4. Expression of a Secreted Protein in Mammalian Cells Using Baculovirus Particles\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Barbara Ann Jardin, Cynthia B. Elias, and Satya Prakash\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e5. Transfection of Difficult-to-Transfect Primary Mammalian Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Oliver Gresch and Ludger Altrogge\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e6. \u003c\/b\u003e\u003cb\u003eStable Protein Expression in Mammalian Cells Using Baculoviruses\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Andreas Lackner, Emanuel Kreidl, Barbara Peter-Vörösmarty, Sabine Spiegl-Kreinecker, Walter Berger, and Michael Grusch\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e7. \u003c\/b\u003e\u003cb\u003eUsing Matrix Attachment Regions to Improve Recombinant Protein Production\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Niamh Harraghy, Montserrat Buceta, Alexandre Regamey, Pierre-Alain Girod, and Nicolas Mermod\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e8. \u003c\/b\u003e\u003cb\u003eControlling Apoptosis to Optimize Yields of Proteins from Mammalian Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Matthew P. Zustiak, Haimanti Dorai, Michael J. Betenbaugh, and Tina M. Sauerwald\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e9. \u003c\/b\u003e\u003cb\u003ePost-Transcriptional Regulatory Elements for Enhancing Transient Gene Expression Levels in Mammalian Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Mariati, Steven C.L. Ho, Miranda G.S. Yap, and Yuansheng Yang\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e10. Converting Monoclonal Antibodies into Fab Fragments for Transient Expression in Mammalian Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Joanne E. Nettleship, Aleksandra Flanagan, Nahid Rahman-Huq, Rebecca Hamer, and Raymond J. Owens\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e11. \u003c\/b\u003e\u003cb\u003eGeneration of High-Expressing Cells by Methotrexate Amplification of Destabilized Dihydrofolate Reductase Selection Marker\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Say Kong Ng\u003c\/p\u003e\u003cp\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e12. \u003c\/b\u003e\u003cb\u003eTools for Co-Producing Multiple Proteins in Mammalian Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Zahra Assur, Wayne A. Hendrickson, and Filippo Mancia\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e13. Identification and Characterization of Protein Glycosylation Using Specific Endo- and Exoglycosidases\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Paula Magnelli, Alicia Bielik, and Ellen Guthrie\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e14. \u003c\/b\u003e\u003cb\u003eStrategies for the Efficient Transfection of CHO-Cells with Plasmid DNA\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Renate Kunert and Karola Vorauer-Uhl\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e15. \u003c\/b\u003e\u003cb\u003eMethods for Constructing Clones for Protein Expression in Mammalian Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Takefumi Sone and Fumio Imamoto\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e16. Optimizing Transient Recombinant Protein Expression in Mammalian Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Ralph F. Hopkins, Vanessa E. Wall, and Dominic Esposito\u003c\/p\u003e\u003ch3\u003eBack Jacket\u003c\/h3\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eThrough all of the recent progress provided by high throughput DNA sequencing technologies, it has become clearer and clearer that the study of proteins and protein organelles will be the key to unlocking our ability to manipulate cells and intervene in human disease. In \u003ci\u003eProtein Expression in Mammalian Cells: Methods and Protocols\u003c\/i\u003e, expert researchers in the field present a compendium of vital techniques to further our knowledge of mammalian protein expression. Written in the highly successful \u003ci\u003eMethods in Molecular Biology(TM)\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003eAuthoritative and concise, \u003ci\u003eProtein Expression in Mammalian Cells: Methods and Protocols\u003c\/i\u003e will aid scientists seeking to delve deeper into our own biology through the medium of other mammalian cells and proteins.\u003cdiv\u003e\n\u003cstrong\u003eNumber of Pages:\u003c\/strong\u003e 276\u003c\/div\u003e\u003cdiv\u003e\n\u003cstrong\u003eDimensions:\u003c\/strong\u003e 0.61 x 10 x 7 IN\u003c\/div\u003e\u003cdiv\u003e\n\u003cstrong\u003eIllustrated:\u003c\/strong\u003e Yes\u003c\/div\u003e\u003cdiv\u003e\n\u003cstrong\u003ePublication Date:\u003c\/strong\u003e August 23, 2016\u003c\/div\u003e","brand":"Books by splitShops","offers":[{"title":"Default Title","offer_id":47431777157298,"sku":"9781493961603","price":194.38,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0770\/3891\/1666\/files\/b2039ea76414eb099dd8c9d7e9215f05.webp?v=1778595348","url":"https:\/\/box.dadyminds.org\/products\/protein-expression-in-mammalian-cells-methods-and-protocols-paperback","provider":"DADYMINDS BOX","version":"1.0","type":"link"}