{"product_id":"synthetic-mrna-production-introduction-into-cells-and-physiological-consequences-paperback","title":"Synthetic Mrna: Production, Introduction Into Cells, and Physiological Consequences - Paperback","description":"\u003cp\u003eby \u003cb\u003eRobert E. Rhoads\u003c\/b\u003e (Editor)\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePart I\u003c\/b\u003e Introduction \u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e1. \u003cb\u003eSynthetic mRNA: Production, Introduction into Cells, and Physiological Consequences\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eRobert E. Rhoads\u003c\/i\u003e Part II Synthesis of mRNA \u003cp\u003e\u003c\/p\u003e2. \u003cb\u003eSynthetic Capped mRNAs for Cap-Specific Photo-Crosslinking Experiments\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eJoanna Kowalska, Franck Martin, and Jacek Jemielity\u003cbr\u003e\u003c\/i\u003e\u003ci\u003e\u003cbr\u003e\u003c\/i\u003e3. \u003cb\u003eEnzymatic Modification of 5'-Capped RNA and Subsequent Labeling by Click Chemistry\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eJosephin M. Holstein, Daniela Stummer, and Andrea Rentmeister\u003cbr\u003e\u003c\/i\u003e\u003ci\u003e\u003cbr\u003e\u003c\/i\u003e4. \u003cb\u003ePreparation of Functional, Fluorescently Labeled mRNA Capped with Anthraniloyl-m\u003csup\u003e7\u003c\/sup\u003eGpppG\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eArtem V. Domashevskiy, David J. Rodriguez, Dilantha Gunawardana, and Dixie J. Goss\u003cbr\u003e\u003c\/i\u003e\u003ci\u003e\u003cbr\u003e\u003c\/i\u003e5. Intronless \u003cb\u003eb\u003c\/b\u003e\u003cb\u003e-Globin Reporter: A Tool for Studying Nuclear RNA Stability Elements\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eJessica A. Brown and Joan A. Steitz\u003cbr\u003e\u003c\/i\u003e\u003ci\u003e\u003cbr\u003e\u003c\/i\u003e6. \u003cb\u003eSynthetic mRNA with Superior Properties that Mimics the Intracellular Fates of Natural Histone mRNA\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eWei Su, Michael K. Slevin, \u003csup\u003e \u003c\/sup\u003eWilliam F. Marzluff, and Robert E. Rhoads\u003cbr\u003e\u003c\/i\u003e\u003ci\u003e\u003cbr\u003e\u003c\/i\u003e7. \u003cb\u003eEngineering WT1-Encoding mRNA to Increase Translational Efficiency in Dendritic Cells\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eDaphné Benteyn, Carlo Heirman, Kris Thielemans, and Aude Bonehill \u003c\/i\u003e\u003cp\u003e\u003c\/p\u003e\u003cb\u003e\u003c\/b\u003ePart III Introduction of Synthetic mRNA into Cells \u003cp\u003e\u003c\/p\u003e8. \u003cb\u003eElectroporation of Alphavirus RNA Translational Reporters into Fibroblastic and Myeloid Cells as a Tool to Study the Innate Immune System\u003cbr\u003e\u003c\/b\u003eChristina L. Gardner, Derek W. Trobaugh, Kate D. Ryman, and William B. Klimstra\u003cbr\u003e\u003ci\u003e\u003cbr\u003e\u003c\/i\u003e9. \u003cb\u003eGMP-Grade mRNA Electroporation of Dendritic Cells for Clinical Use\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eJudith Derdelinckx, Zwi N. Berneman, and Nathalie Cools\u003cbr\u003e\u003c\/i\u003e\u003ci\u003e\u003cbr\u003e\u003c\/i\u003e10. \u003cb\u003eLarge Scale mRNA Transfection of Dendritic Cells by Electroporation in Continuous Flow Systems\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eDavid Selmeczi, Thomas Steen Hansen, Özcan Met, Inge Marie Svane, and Niels B. Larsen\u003cbr\u003e\u003c\/i\u003e\u003ci\u003e\u003cbr\u003e\u003c\/i\u003e11. \u003cb\u003eFLT3 Ligand as a Molecular Adjuvant for Naked RNA Vaccines\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eSebastian Kreiter, Mustafa Diken, Abderraouf Selmi, Jutta Petschenka, Özlem Türeci, and Ugur Sahin\u003cbr\u003e\u003c\/i\u003e\u003cbr\u003e12. \u003cb\u003eTransfecting Human Monocytes with RNA\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eJens Dannull and Smita K. Nair\u003cbr\u003e\u003c\/i\u003e\u003ci\u003e\u003cbr\u003e\u003c\/i\u003e13.In Vitro Synthesis, Delivery and Bioavailability of Exogenous mRNA in Gene Transfer Mediated by \u003ci\u003ePiggyBac\u003c\/i\u003e Transposition\u003cbr\u003e\u003ci\u003eSolenne Bire, Nicole Ishac, and Florence Rouleux-Bonnin\u003cbr\u003e\u003c\/i\u003e\u003ci\u003e\u003cbr\u003e\u003c\/i\u003e14. Transfection of Human Keratinocytes with Nucleoside-Modified mRNA Encoding CPD-Photolyase to Repair DNA Damage\u003cbr\u003eGábor Boros, Katalin Karikó, Hiromi Muramatsu, Edit Miko, Eszter Emri, Csaba Hegedűs, Gabriella Emri, and Éva Remenyik \u003cp\u003e\u003c\/p\u003ePart IV Alteration of Cellular Function with Synthetic mRNA\u003cbr\u003e\u003cb\u003e\u003cbr\u003e15. \u003cb\u003eDelivery of Synthetic mRNA Encoding FOXP3 Antigen into Dendritic Cells for Inflammatory Breast Cancer Immunotherapy\u003cbr\u003e\u003c\/b\u003e\u003c\/b\u003e\u003ci\u003eGayathri R. Devi and Sritama Nath\u003cbr\u003e\u003c\/i\u003e\u003ci\u003e\u003c\/i\u003e16. \u003cb\u003eImmune Monitoring using mRNA-Transfected Dendritic Cells\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eTroels Holz Borch, Inge Marie Svane, and Özcan Met\u003cbr\u003e\u003c\/i\u003e\u003ci\u003e\u003cbr\u003e\u003c\/i\u003e17. \u003cb\u003eTransfection of Tumor-Infiltrating T Cells with mRNA Encoding CXCR2\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eManja Idorn, Per thor Straten, Inge Marie Svane, and Özcan Met\u003cbr\u003e\u003c\/i\u003e\u003ci\u003e\u003cbr\u003e\u003c\/i\u003e18. \u003cb\u003emRNA Electroporation of Dendritic Cells with WT1, Survivin and TriMix (A Mixture of caTLR4, CD40L, and CD70)\u003cbr\u003e\u003c\/b\u003eAn Coosemans, Sandra Tuyaerts, Kim Morias, Jurgen Corthals, Carlo Heirman, Kris Thielemans, Stefaan W. Van Gool, Ignace Vergote, and Frédéric Aman\u003ch3\u003eBack Jacket\u003c\/h3\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eThis volume presents detailed laboratory protocols for \u003ci\u003ein vitro\u003c\/i\u003e synthesis of mRNA with favorable properties, its introduction into cells by a variety of techniques, and the measurement of physiological and clinical consequences such as protein replacement and cancer immunotherapy. Synthetic techniques are described for structural features in mRNA that provide investigational tools such as fluorescence emission, click chemistry, photo-chemical crosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reduced activation of the innate immune response. Protocols are described for clinical applications such as large-scale transfection of dendritic cells, production of GMP-grade mRNA, redirecting T cell specificity, and use of molecular adjuvants for RNA vaccines. Written in the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003cbr\u003e\u003ci\u003e\u003cbr\u003eSynthetic mRNA: Production, Introduction into Cells, and Physiological Consequences \u003c\/i\u003eis a valuable and cutting-edge resource for both laboratory investigators and clinicians interested in this powerful and rapidly evolving technology. \u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cdiv\u003e\n\u003cstrong\u003eNumber of Pages:\u003c\/strong\u003e 320\u003c\/div\u003e\u003cdiv\u003e\n\u003cstrong\u003eDimensions:\u003c\/strong\u003e 0.7 x 10 x 7 IN\u003c\/div\u003e\u003cdiv\u003e\n\u003cstrong\u003eIllustrated:\u003c\/strong\u003e Yes\u003c\/div\u003e\u003cdiv\u003e\n\u003cstrong\u003ePublication Date:\u003c\/strong\u003e May 26, 2018\u003c\/div\u003e","brand":"Books by splitShops","offers":[{"title":"Default Title","offer_id":47431845281970,"sku":"9781493980987","price":194.38,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0770\/3891\/1666\/files\/7418ea270e532b1abc0b032982de742b.webp?v=1778596381","url":"https:\/\/box.dadyminds.org\/products\/synthetic-mrna-production-introduction-into-cells-and-physiological-consequences-paperback","provider":"DADYMINDS BOX","version":"1.0","type":"link"}